Yes in most cases for short periods of time, I would only do it for an emergency as the tube will heat up and the tubeless bead does not always seat as good as it should. In addition you might have have to mount your own tires, tire shops may not be willing to do it for you, this is especially true of the big shops due to liability and the fact that tubed tires require a different mounting tool. If you mount them yourself be careful not to burst the tube, if you do burst the tube you'll never know as the tire itself will hold air, you'll only find out when you get a flat.
One thing to think about is the condition of the tire after its burst. If you drive over a nail or something equally small you can fix it with a vulcanizing (sp?) rubber plug. If the damage is so great that a plug or patch cannot be used you will not be able to run a tube, either the tube will protrude through the hole in the tire and burst, or the tire itself will burst the tube.
To get it to reliably work you would have to pull the tire from the rim, use bailing wire to stitch up the gash in the structure and put a good sized patch on the inside. Thats a lot of work and you can probably make it airtight without a spare.
I would get a set of tire irons and wedges and practice mounting/unmounting them by hand, then just take additional tires with you. Its a good skill to have regardlessly.
